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docs:gaussian_beam [2019/02/09 00:00] Jon Daniels |
docs:gaussian_beam [2019/05/07 23:18] (current) Jon Daniels |
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| For a typical diSPIM setup, NA_illum = 0.366 * iris_D / EFL where | For a typical diSPIM setup, NA_illum = 0.366 * iris_D / EFL where | ||
| * 0.366 is the half the total magnification between iris and back focal plane | * 0.366 is the half the total magnification between iris and back focal plane | ||
| - | * iris_D is the iris diameter | + | * iris_D is the iris diameter |
| * EFL is the effective focal length of the objective, e.g. 5mm for Nikon 40x, 9mm for Olympus 20x, and 12mm for the cleared tissue objective @ RI~1.45 | * EFL is the effective focal length of the objective, e.g. 5mm for Nikon 40x, 9mm for Olympus 20x, and 12mm for the cleared tissue objective @ RI~1.45 | ||
