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hardware:environmental_control [2017/10/26 18:44]
Jon Daniels |
hardware:environmental_control [2020/11/19 08:03] (current)
Tokai Hit Co. Ltd |
| For long-term imaging of some samples the environment must be tightly controlled, including temperature, humidity, and gas concentrations (e.g. CO2). | For long-term imaging of some samples the environment must be tightly controlled, including temperature, humidity, and gas concentrations (e.g. CO2). |
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| Temperature can either be controlled locally around the sample or the bulk of the microscope can be enclosed and kept at a constant temperature. ASI makes an incubator enclosure that fits around the entire diSPIM on RAMM. Incubator boxes for diSPIM on other inverted microscopes are available from by 3rd parties. Tokai Hit makes an insert with objective heaters for temperature control without requiring an enclosure, description {{ asi_heating_system.pdf | here }}. | Temperature can either be controlled locally around the sample or the bulk of the microscope can be enclosed and kept at a constant temperature. ASI makes an incubator enclosure that fits around the entire diSPIM on RAMM. Incubator boxes for diSPIM on other inverted microscopes are available from by 3rd parties. |
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| | Tokai Hit makes an insert with objective heaters for temperature control without requiring an enclosure, description {{ asi_heating_system.pdf | here }}. Besides it, they developed an incubator based system which can maintain temperature, CO2, and humidity with a closed environment during imaging, description {{ :hardware:asi_incubator_system.pdf | here }}. |
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| <imgcaption RAMM_Incubator_model| Model of a diSPIM on RAMM in incubator enclosure.>{{:hardware:incubator_dispim.png?200|diSPIM on RAMM in incubator enclosure}} </imgcaption> <imgcaption RAMM_Incubator_photo| Photo of a diSPIM on RAMM in incubator enclosure.>{{:hardware:dispim_in_incubator.jpg?300|diSPIM on RAMM in incubator enclosure}} </imgcaption> | <imgcaption RAMM_Incubator_model| Model of a diSPIM on RAMM in incubator enclosure.>{{:hardware:incubator_dispim.png?200|diSPIM on RAMM in incubator enclosure}} </imgcaption> <imgcaption RAMM_Incubator_photo| Photo of a diSPIM on RAMM in incubator enclosure.>{{:hardware:dispim_in_incubator.jpg?300|diSPIM on RAMM in incubator enclosure}} </imgcaption> |
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| <imgcaption Tokai_Hit_system | Tokai Hit thermal control system>{{:hardware:asi_heating_system.jpg?300| Tokai Hit thermal control system for diSPIM}} </imgcaption> | <imgcaption image1|>{{:hardware:asi_incubator_system_p1.jpg?200|}}</imgcaption> |
| | <imgcaption image1|>{{:hardware:asi_incubator_system_p2.jpg?200|}}</imgcaption> |
| | <imgcaption image1|>{{:hardware:asi_incubator_system_2.jpg?200|}}</imgcaption> |
| | <imgcaption image1|>{{:hardware:asi_incubator_system_3.jpg?200|}}</imgcaption> |
